Irradiated 3T3-msCD40L Feeder Cells
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Irradiated 3T3-msCD40L Feeder Cells

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Irradiated 3T3-msCD40L Feeder Cells

Irradiated 3T3-msCD40L Feeder Cells

Catalog # C6003

For in vitro use only

Alternative name

3T3-msCD40L, 3T3-CD40L treated by irradiation


3T3-CD40L is a mouse fibroblast cell line that secretes mouse CD40L. 3T3-CD40L cells can be co-cultured with B cells to aid B cell expansion. Irradiated 3T3-msCD40L cells are treated by irradiation, and ready for feeding B cell culture.

Cell Type

Mouse 3T3 cell line expression CD40L


1 vials (for 2 96-well plates)



Biosafety Level 



Adherent Cells, like fibroblast cells

Maintenance Tips

Irradiated 3T3-CD40L cells are used for feeding B cell for once, and cannot be maintained for long term.

Propagation Medium

DMEM with 10% FBS, 1% L-glutamine, and 0.1% gentamicin.


J. Huang, N. A. Doria-Rose, N. S. Longo, L. Laub, C.-L. Lin, E. Turk, B. H. Kang, S. A. Migueles, R. T. Bailer, J. R. Mascola, M. Connors, Isolation of Human Monoclonal Antibodies from Peripheral Blood B Cells. Nat. Protoc. 8, 1907-1915 (2013)


For laboratory research only. Not for clinical applications.

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